Te seed of Nigella sativa (N. sativa) has been used in diferent civilization around the world for centuries to treat various animal and human ailments. So far, numerous studies demonstrated the seed of Nigella sativa and its main active constituent, thymoquinone, to be medicinally very efective against various illnesses including diferent chronic illness: neurological and mental illness, cardiovascular disorders, cancer, diabetes, infammatory conditions, and infertility as well as various infectious diseases due to bacterial, fungal, parasitic, and viral infections. In spite of limited studies conducted so far, the promising efcacy of N. sativa against HIV/AIDS can be explored as an alternative option for the treatment of this pandemic disease afer substantiating its full therapeutic efcacy. Moreover, the strong antioxidant property of this valued seed has recently gained increasing attention with regard to its potential role as dietary supplement with minimal side efects. Besides, when combined with diferent conventional chemotherapeutic agents, it synergizes their efects resulting in reducing the dosage of concomitantly used drugs with optimized efcacy and least and/or no toxicity. A number of pharmaceutical and biological properties have been ascribed to seeds of N. sativa. Te present review focuses on the profle of high-value components along with traditional medicinal and biological principles of N. sativa seed and its oil so as to explore functional food and nutraceutical potential of this valued herb.